How To Light Test Tube For Laboratory?

It is imperative that all specimens are protected from light. Wrapping the specimen in aluminum foil or paper towel or any other light-resistant material is a good idea.

Table of contents

What Color Tubes Go With What Lab Tests?

Tube cap color


Common laboratory tests

Lavender or pink

Potassium EDTA

Hematology and blood bank


Sodium fluoride, and sodium or potassium oxalate

Glucose (especially when testing will be delayed), blood alcohol, lactic acid

What Order Do You Draw Lab Tubes?

  • Culture tubes or bottles for blood.
  • The sodium citrate tube (blue closure) is made of sodium citrate.
  • Tubes of serum, including those with clot activators and gels (red, red-speckled, gold closures).
  • The tube should be either dark green, light green, or speckled green (e.g., dark green, light green, speckled green).
  • How Many Times Do You Invert Blood Tubes?

    In order to ensure adequate anticoagulation of the specimen after it has been filled with blood, invert the tube 8-10 times after it has been filled with blood.

    What Color Tube For Lab Draws?




    Light Blue

    3.2% Sodium Citrate

    4.5 mL

    Gold Top (Serum Separator, “SST”)

    Contains separating gel and clot activator

    6.0 mL

    Light Blue – Yellow Label on Tube


    2.0 mL


    Sodium heparin(100 USP Units)

    5.0 mL

    What Are The Types Of Test Tubes?

    Glass, plastic, metal, and ceramic are some of the materials used in test tubes. The most common materials are glass and plastic, while metal and ceramic are less common. Glass and plastic test tubes can be classified into several types.

    What Is The Light Blue Test Tube Used For?

    The U. U.S. Food and Drug Administration (FDA) is aware of the issue. Due to an increase in demand, sodium citrate blood specimen collection tubes, also known as light blue top tubes (hereafter “sodium citrate tubes”), used for coagulation (blood clotting), have been experiencing significant interruptions in supply.

    What Are The Different Colored Blood Tubes For?

  • A clot activator and a special gel are included in the Gold version to separate the serum from the cells.
  • A purple color indicates that it contains a clot blocker and is used for complete blood counts (CBC).
  • A plasma test is conducted using Green & Light Blue.
  • What Labs Can Go In The Same Tube?

    CBC, SED Rate (ESR), Reticulocyte can be drawn in the same Lavender (Purple) top tube. You should draw an extra lavender on each Glycohemoglobin and BNP. A glucose test can be performed with EDTA sodium fluoride (serum tube). Blood plasma samples will be taken from oxalate and EDTA anticoagulants.

    What Color Tube Is Used For A Cbc?

    A lavender-top tube or microcollection tube is required for the collection of EDTA. You can also draw an additional blue-top (sodium citrate) tube if you want EDTA platelets to clump only.

    What Test Must Be Protected From Light?

    Test ID

    Test Name


    Bilirubin Total, S


    Bilirubin, BF


    Bilirubin, Direct


    Bilirubin, Random, U

    Which Of These Test Samples Are Light Sensitive?

    Bilbin is one of the light-sensitive analytes. The specimen must be protected from light by wrapping the tube in aluminum foil, or by using an amber-colored tube. In addition to vitamin B12, carotene, folate, and urine porphyrin, there are other light-sensitive analytes.

    Does Vitamin B1 Need To Be Protected From Light?

    The VITAMIN B1 (THIAMINE) is a blood, LC/MS/MS 2 mL EDTA whole blood that is protected from light. A minimum of 1 mL of EDTA is required to protect whole blood from light. The following notes are special: Protect the specimen from light.

    Should Vitamin B12 Be Protected From Light?

    It was demonstrated that serum vitamin B12 was highly unstable, so specimens should be frozen if not analyzed immediately. In the absence of a 4 hour sample analysis, light protection is necessary.

    How Do You Remember The Order Of Blood Draw Tubes?

    A mnemonic is sometimes used by phlebotomists to help them remember. Market Lab shared some very funny mnemonics, such as “Studious Boy’s Rarely Get Low Grades” which refers to Sterile, Blue, Green, Lavender, and Grey.

    Does It Matter What Order You Draw Labs?

    As lyophilized anticoagulants become more common, order of draw is no longer necessary. In the absence of order of draw, serum samples will be contaminated with K EDTA on a regular basis. The difference between K and EDTA tubes can be determined by modern lab equipment, so ordering the draw is not important.

    How Many Times Do You Invert The Black Tube?

    Ensure that the mixture is well mixed by inverting the tube at least eight times.

    How Many Times Do You Invert A Blue Tube?

    The EDTA tubes in dark blue are used for whole blood analysis of trace elements. To ensure proper mixing with the anticoagulant, invert the tube six to eight times after collection.

    How Many Times Do You Invert A Green Top Tube?

    The anticoagulant sodium heparin or lithium heparin is in green (Sodium or Lithium Heparin). Blood, whole blood, or bone marrow specimens can be prepared with this tube. It is recommended that the tube be inverted 8-10 times after collection to prevent clotting.

    How Long Is Unspun Blood Good For?

    Based on these results, it appears that tubes stored unspun at room temperature for up to 8 hours after blood collection can be reliably evaluated for PT/INR, aPTT, fibrinogen, FV, and D-dimer. FVIII should be delayed by at least six hours.

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